Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditions
Articolo
Data di Pubblicazione:
2019
Citazione:
Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditions / M. Colombo, M.G. Morselli, M.R. Tavares, M. Apparicio, G.C. Luvoni. - In: ANIMALS. - ISSN 2076-2615. - 9:6(2019 Jun), pp. 329.1-329.13.
Abstract:
Cryoinjuries severely affect the competence of vitrified oocytes (VOs) to develop into
embryos after warming. The use of culture conditions that provide physical and chemical support
and resemble the in vivo microenvironment in which oocytes develop, such as 3D scaffolds and
coculture systems, might be useful to improve VOs outcomes. In this study, an enriched culture
system of 3D barium alginate microcapsules was employed for the in vitro embryo production of
domestic cat VOs. Cryotop vitrified-warmed oocytes were in vitro matured for 24 h in the 3D system
with or without fresh cumulus-oocyte complexes (COCs) in coculture, whereas a control group of
VOs was cultured in traditional 2D microdrops of medium. After in vitro fertilization, presumptive
embryos were cultured in 3D or 2D systems according to the maturation conditions. Vitrified oocytes
were able to mature and develop into embryos in 3D microcapsules (17.42 +/- 11.83%) as well as in
2D microdrops (14.96 +/- 8.80%), but the coculture with companion COCs in 3D resulted in similar
proportions of VOs embryo development (18.39 +/- 16.67%; p = 1.00), although COCs presence allowed
for blastocyst formation (0.95 +/- 2.52%). In conclusion, embryos until late developmental stages were
obtained from cat VOs, and 3D microcapsules were comparable to 2D microdrops, but improvements
in post-warming conditions are still needed.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
alginate microcapsule; blastocyst; cryopreservation; feline; in vitro culture; three-dimensional
Elenco autori:
M. Colombo, M.G. Morselli, M.R. Tavares, M. Apparicio, G.C. Luvoni
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