Data di Pubblicazione:
2007
Citazione:
Bioremediation and monitoring of aromatic-polluted habitats / V. Andreoni, L. Gianfreda. - In: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. - ISSN 0175-7598. - 76:2(2007), pp. 287-308.
Abstract:
Bioremediation may restore contaminated soils
through the broad biodegradative capabilities evolved by
microorganisms towards undesirable organic compounds.
Understanding bioremediation and its effectiveness is
rapidly advancing, bringing available molecular approaches
for examining the presence and expression of the key genes
involved in microbial processes. These methods are
continuously improving and require further development
and validation of primer- and probe-based analyses and
expansion of databases for alternative microbial markers.
Phylogenetic marker approaches provide tools to determine
which organisms are present or generally active in a community;
functional gene markers provide only information
concerning the distribution or transcript levels (deoxyribonucleic
acid [DNA]- or messenger ribonucleic acid [mRNA]-
based approaches) of specific gene populations across
environmental gradients. Stable isotope probing methods
offer great potential to identify microorganisms that metabolize
and assimilate specific substrates in environmental
samples, incorporating usually a rare isotope (i.e., 13C) into
their DNA and RNA. DNA and RNA in situ characterization
allows the determination of the species actually involved in
the processes being measured. DNA microarrays may
analyze the expression of thousands of genes in a soil
simultaneously. A global analysis of which genes are being
expressed under various conditions in contaminated soils will
reveal the metabolic status of microorganisms and indicate
environmental modifications accelerating bioremediation.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
V. Andreoni, L. Gianfreda
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