Evaluation of neuroactive steroid levels by liquid chromatography-tandem mass spectrometry in central and peripheral nervous system : effect of diabetes
Articolo
Data di Pubblicazione:
2008
Citazione:
Evaluation of neuroactive steroid levels by liquid chromatography-tandem mass spectrometry in central and peripheral nervous system : effect of diabetes / D. Caruso, S. Scurati, O. Maschi, L. DE ANGELIS, I. Roglio, S. Giatti, L.M. Garcia Segura, C. Melcangi. - In: NEUROCHEMISTRY INTERNATIONAL. - ISSN 0197-0186. - 52:4-5(2008), pp. 560-568. [10.1016/j.neuint.2007.06.004]
Abstract:
The nervous system is a target for physiological and protective effects of neuroactive steroids. Consequently, the assessment of their levels in
nervous structures under physiological and pathological conditions is a top priority. To this aim, identification and quantification of pregnenolone
(PREG), progesterone (PROG), dihydroprogesterone (DHP), tetrahydroprogesterone (THP), testosterone (T), dihydrotestosterone (DHT), 5aandrostan-
3a, 17b-diol (3a-diol), 17a- and 17b-estradiol (17a-E and 17b-E) by liquid chromatography and tandem mass spectrometry (LC–MS/
MS) has been set up. After validation, this method was applied to determine the levels of neuroactive steroids in central (i.e., cerebral cortex,
cerebellum and spinal cord) and peripheral (i.e., brachial nerve) nervous system of control and diabetic rats. In controls only the brachial nerve had
detectable levels of all these neuroactive steroids. In contrast, 17a-E in cerebellum, 17a-E, 17b-E, DHP and THP in cerebral cortex, and 17a-E,
17b-E and DHP in spinal cord were under the detection limit. Diabetes, induced by injection with streptozotocin, strongly affected the levels of
some neuroactive steroids. In particular, the levels of PREG, PROG and T in cerebellum, of PROG, T and 3a-diol in cerebral cortex, of PROG,
DHTand 3a-diol in spinal cord and of PREG, DHP, THP, T, DHTand 3a-diol in brachial nerve were significantly decreased. In conclusion, the data
here reported demonstrate that the LC–MS/MS method allows the assessment of neuroactive steroids in the nervous system with high sensitivity
and specificity and that diabetes strongly affects their levels, providing a further basis for new therapeutic tools based on neuroactive steroids aimed
at counteracting diabetic neuropathy.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Central nervous system; Neuroprotection; Peripheral nervous system; Rat; Steroid level; Streptozotocin
Elenco autori:
D. Caruso, S. Scurati, O. Maschi, L. DE ANGELIS, I. Roglio, S. Giatti, L.M. Garcia Segura, C. Melcangi
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