Data di Pubblicazione:
2018
Citazione:
A qPCR-based protocol to quantify DSB resection / M. Ferrari, S. Twayana, F. Marini, A. Pellicioli (METHODS IN MOLECULAR BIOLOGY). - In: Genome Instability : Methods and Protocols / [a cura di] M. Muzi-Falconi, G. W Brown. - [s.l] : Humana, 2018. - ISBN 9781493973057. - pp. 119-129
Abstract:
The nucleolytic degradation of the 5′-ending strand of a Double-Strand DNA break (DSB) is necessary to initiate homologous recombination to correctly repair the break. This process is called DNA end resection and it is finely regulated to prevent genome rearrangements. Here, we describe a protocol to quantify DSB resection rate by qPCR, which could be applied to every organisms whenever the break site and its flanking region sequences are known.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
DNA end resection; Double-strand breaks; Single-strand DNA; qPCR
Elenco autori:
M. Ferrari, S. Twayana, F. Marini, A. Pellicioli
Link alla scheda completa:
Titolo del libro:
Genome Instability : Methods and Protocols