Epithelial-To-Mesenchymal Transition Markers and CD44 Isoforms Are Differently Expressed in 2D and 3D Cell Cultures of Prostate Cancer Cells
Articolo
Data di Pubblicazione:
2019
Citazione:
Epithelial-To-Mesenchymal Transition Markers and CD44 Isoforms Are Differently Expressed in 2D and 3D Cell Cultures of Prostate Cancer Cells / F. Fontana, M. Raimondi, M. Marzagalli, M. Sommariva, P. Limonta, N. Gagliano. - In: CELLS. - ISSN 2073-4409. - 8:2(2019 Feb 11), pp. 143.1-143.17. [10.3390/cells8020143]
Abstract:
Three-dimensional (3D) cell cultures allow the mimic of functions of living tissues and
provide key information encoded in tissue architecture. Considered the pivotal role of epithelial-tomesenchymal
transition (EMT) in carcinoma progression, including prostate cancer (PCa), we
aimed at investigating the effect of the 3D arrangement on the expression of some key markers of
EMT in cultured human prostate cancer (PCa) cells, to better understand PCa cell behavior. PC3 and
DU145 PCa cells were cultured in RPMI cell culture medium either in 2D-monolayers or in 3Dspheroids.
The main EMT markers E-cadherin, N-cadherin, α-smooth muscle actin (αSMA),
vimentin, Snail, Slug, Twist and Zeb1 were evaluated by confocal microscopy, real-time PCR and
Western blot. Confocal microscopy revealed that E-cadherin was similarly expressed at the cell
boundaries on the plasma membrane of PCa cells grown in 2D-monolayers, as well as in 3Dspheroids,
but resulted up-regulated in 3D-spheroids, compared to 2D-monolayers, at the mRNA
and protein level. Moreover, markers of the mesenchymal phenotype were expressed at very low
levels in 3D-spheroids, suggesting important differences in the phenotype of PCa cells grown in 3Dspheroids
or in 2D-monolayers. Considered as a whole, our findings contribute to a clarification of
the role of EMT in PCa and confirm that a 3D cell culture model could provide deeper insight into
the understanding of the biology of PCa.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
epithelial-to-mesenchymal transition; 3D-spheroids; E-cadherin; CD44; prostate cancer
Elenco autori:
F. Fontana, M. Raimondi, M. Marzagalli, M. Sommariva, P. Limonta, N. Gagliano
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