Response of methanogen populations to organic load increase during anaerobic digestion of olive mill wastewater
Articolo
Data di Pubblicazione:
2006
Citazione:
Response of methanogen populations to organic load increase during anaerobic digestion of olive mill wastewater / A. Rizzi, M. Zucchi, S. Borin, M. Marzorati, C. Sorlini, D. Daffonchio. - In: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY. - ISSN 0268-2575. - 81:9(2006 Sep), pp. 1556-1562. [10.1002/jctb.1558]
Abstract:
Process performances of an upflow anaerobic filter treating olive mill wastewater and the response of methanogenic Archaea to increasing volumetric organic load (VOL) were studied. At a VOL of 15 g chemical oxygen demand (COD) L-1 day 90% of the influent COD was removed. Following a VOL increase from 6 to 15 g COD L-1 day(-1), the polymerase chain reaction (PCR) titre of hydrogenotrophic Methanobacterium, determined by magnetic capture of the target DNA and group-specific PCR based on the 16S rRNA gene, decreased from 10(11) to 10(8) cells g(-1) sludge, while that of Methanomicrobiaceae and relatives increased from 10(4) to 10(6) cells g(-1) sludge. Methanosaeta-like acetoclastic methanogens were less affected by VOL variation and dominated at high VOL with a 16S rRNA gene PCR titre of 10(9) cells g(-1) sludge. Single-strand conformation polymorphism analysis of the PCR-amplified archaeal 16S rRNA gene showed a stable band pattern, indicating that VOL variation affected the methanogen PCR titre but not the archaeal community structure. (c) 2006 Society of Chemical Industry.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Olive mill wastewater ; anaerobic digestion ; methanogens ; organic load ; PCR monitoring ; SSCP
Elenco autori:
A. Rizzi, M. Zucchi, S. Borin, M. Marzorati, C. Sorlini, D. Daffonchio
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