Role of gangliosides in the association of ErbB2 with lipid rafts in mammary epithelial HC11 cells
Articolo
Data di Pubblicazione:
2006
Citazione:
Role of gangliosides in the association of ErbB2 with lipid rafts in mammary epithelial HC11 cells / E. Sottocornola, R. Misasi, V. Mattei, L. Ciarlo, R. Gradini, T. Garofalo, B. Berra, I. Colombo, M. Sorice. - In: THE FEBS JOURNAL. - ISSN 1742-464X. - 273:8(2006), pp. 1821-1830. [10.1111/j.1742-4658.2006.05203.x]
Abstract:
We analyzed the role of gangliosides in the association of the ErbB2 receptor
tyrosine-kinase (RTK) with lipid rafts in mammary epithelial HC11
cells. Scanning confocal microscopy experiments revealed a strict ErbB2–
GM3 colocalization in wild-type cells. In addition, analysis of membrane
fractions obtained using a linear sucrose gradient showed that ErbB2, epidermal
growth factor receptor (EGFR) and Shc-p66 (proteins correlated
with the ErbB2 signal transduction pathway) were preferentially enriched
in lipid rafts together with gangliosides. Blocking of endogenous
ganglioside synthesis by (+⁄ –)-threo-1-phenyl-2-decanoylamino-3-morpholino-
1-propanol hydrochloride ([D]-PDMP) induced a drastic cell-surface
redistribution of ErbB2, EGFR and Shc-p66, within the Triton-soluble
fractions, as revealed by linear sucrose-gradient analysis. This redistribution
was partially reverted when exogenous GM3 was added to gangliosidedepleted
HC11 cells. The results point out the key role of ganglioside GM3
in retaining ErbB2 and signal-transduction-correlated proteins in lipid
rafts.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
E. Sottocornola, R. Misasi, V. Mattei, L. Ciarlo, R. Gradini, T. Garofalo, B. Berra, I. Colombo, M. Sorice
Link alla scheda completa: