Data di Pubblicazione:
1995
Citazione:
The Escherichia coli heat shock response and bacteriophage lambda development / A. Polissi, L. Goffin, C. Georgopoulos. - In: FEMS MICROBIOLOGY REVIEWS. - ISSN 0168-6445. - 17:1-2(1995 Aug), pp. 159-169. ((Intervento presentato al convegno EMBO-FEMS Meeting on Bacterial Viruses - Molecular Biology and Biotechnology tenutosi a Gargnano nel 1994.
Abstract:
The Escherichia coli/bacteriophage lambda genetic interaction system has been used to uncover the existence of various biological machines. The starting point of all these studies was the isolation and characterization of E. coli mutants that blocked lambda growth, and the corresponding lambda compensatory mutations. In this manner, the lambda N-promoted transcriptional anti-termination machine was discovered composed of the NusA/NusB/NusE/NusG host proteins. In addition, the DnaK and GroEL chaperone machines were discovered composed of DnaK/DnaJ/GrpE and GroES/GroEL heat shock proteins. The individual members of the DnaK and GroEL chaperone machines have been conserved throughout evolution in both function and structure. Their biological roles include a direct involvement in lambda DNA replication and morphogenesis, the protection of proteins from aggregation, the disaggregation of various protein aggregates, the manipulation of protein structure and function, as well as the autoregulation of the heat shock response. The evolution of lambda to extensively rely on the status of the heat shock response of E. coli is likely linked to its lytic versus lysogenic choice of lifestyle. The bacteriophage T4 gp31 protein has been purified and shown to substitute for many of GroES' co-chaperonin activities.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Groel chaperone machine; DNAK chaperone machine; heat shock response; bacteriophage-lambda; bacteriophage-T4 GP31 co-chaperone; regulation response of heat shock
Elenco autori:
A. Polissi, L. Goffin, C. Georgopoulos
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