Use of a PTFE Micro-Bioreactor to Promote 3D Cell Rearrangement and Maintain High Plasticity in Epigenetically Erased Fibroblasts
Articolo
Data di Pubblicazione:
2019
Citazione:
Use of a PTFE Micro-Bioreactor to Promote 3D Cell Rearrangement and Maintain High Plasticity in Epigenetically Erased Fibroblasts / G. Pennarossa, E.F.M. Manzoni, S. Ledda, M. deEguileor, F. Gandolfi, T. Brevini. - In: STEM CELL REVIEWS. - ISSN 1550-8943. - 15:1(2019 Feb), pp. 82-92. [10.1007/s12015-018-9862-5]
Abstract:
Phenotype definition is driven by epigenetic mechanisms as well as directly influenced by the cell microenvironment and by biophysical signals deriving from the extracellular matrix. The possibility to interact with the epigenetic signature of an adult mature cell, reversing its differentiated state and inducing a short transient high plasticity window, was previously demonstrated. In parallel, in vitro studies have shown that 3D culture systems, mimicking cell native tissue, exert significant effects on cell behavior and functions. Here we report the production of “PTFE micro-bioreactors” for long-term culture of epigenetically derived high plasticity cells. The system promotes 3D cell rearrangement, global DNA demethylation and elevated transcription of pluripotency markers, that is dependent on WW domain containing transcription regulator 1 (TAZ) nuclear accumulation and SMAD family member 2 (SMAD2) co-shuttling. Our findings demonstrate that the use of 3D culture strategies greatly improves the induction and maintenance of a high plasticity state.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Epigenetic erasing; High plasticity; Micro-bioreactor; PTFE; SMAD2; TAZ; Cell Biology; Cancer Research
Elenco autori:
G. Pennarossa, E.F.M. Manzoni, S. Ledda, M. Deeguileor, F. Gandolfi, T. Brevini
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