Identification and characterization of the a-CA in the outer membrane vesicles produced by Helicobacter pylori
Articolo
Data di Pubblicazione:
2019
Citazione:
Identification and characterization of the a-CA in the outer membrane vesicles produced by Helicobacter pylori / M. Roncia, S. Del Prete, V. Puca, S. Carradori, V. Carginale, R. Muraro, G. Mincione, A. Aceto, F. Sisto, C.T. Supuran, R. Grande, C. Capasso. - In: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY. - ISSN 1475-6366. - 34:1(2019 Jan 01), pp. 189-195. [10.1080/14756366.2018.1539716]
Abstract:
The genome of Helicobacter pylori encodes for carbonic anhydrases (CAs, EC 4.2.1.1) belonging to the aand b-CA classes, which together with urease, have a pivotal role in the acid acclimation of the microorganism within the human stomach. Recently, in the exoproteome of H. pylori, a CA with no indication of the corresponding class was identified. Here, using the protonography and the mass spectrometry, a CA belonging to the a-class was detected in the outer membrane vesicles (OMVs) generated by planktonic and biofilm phenotypes of four H. pylori strains. The amount of this metalloenzyme was higher in the planktonic OMVs (pOMVs) than in the biofilm OMVs (bOMVs). Furthermore, the content of a-CA increases over time in the pOMVs. The identification of the a-CA in pOMVs and bOMVs might shed new light on the role of this enzyme in the colonization, survival, persistence, and pathogenesis of H. pylori
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Carbonic anydrases; Helicobacter pylori; outer membrane vesicles (OMVs); biofilm; protonography; mass spectrometry
Elenco autori:
M. Roncia, S. Del Prete, V. Puca, S. Carradori, V. Carginale, R. Muraro, G. Mincione, A. Aceto, F. Sisto, C.T. Supuran, R. Grande, C. Capasso
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