Genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from the Autonomous Province of Trento, Italy
Articolo
Data di Pubblicazione:
2007
Citazione:
Genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from the Autonomous Province of Trento, Italy / E. Pecchioli, H.C. Hauffe, V. Tagliapietra, C. Bandi, C. Genchi, A. Rizzoli. - In: INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY. - ISSN 1438-4221. - 297:1(2007), pp. 53-59.
Abstract:
Sequences of the variable intergenic spacer region 5S (rrfA) 23S (rrlB) rRNA were used to identify Borrelia genospecies present in Ixodes ricinus nymphs collected from the Lamar Lakes area of the Province of Trento, Italy (overall
prevalence=6.3%). Four genospecies were identified, one for the first time in this Province (B. valaisiana), and three which have been noted previously (B. afzelii, B. garinii, and B. burgdorferi s.s.). In order to compare the genetic variability of these genospecies in Trento with that at a European level, our 21
sequences (15 new haplotypes) and all appropriate European Borrelia sequences registered in GenBank (up to the end of 2004) were subjected to a phylogeneticanalysis (for a total of 73 sequences and 43 haplotypes). Clusters of sequences
representing the five main European genospecies (afzelii, garinii, burgdorferi s.s., valaisiana, lusitaniae) are well-supported. At least two other groups of haplotypes (genospecies) are suggested by our analysis; moreover, divergent
evolution may be occurring in several genospecies. The maximum uncorrected
pairwise differences between sequences within genospecies ranges from 1.5% (B. burgdorferi s.s.), to 2.3% (B. garinii and B. valaisiana) to 4.7% (B. afzelii), and are not correlated with geographical distribution. Within the Province of
Trento, these values for the same genospecies are 1.5%, 2.3%, 0.9%, 1.9%, respectively. These high mutation rates within genospecies suggest that the
sequencing of haplotypes should continue if we are to fully understand and monitor the evolution and epidemiology of Borrelia.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Borrelia; Genospecies; Italy; Ixodes; Lyme borreliosis
Elenco autori:
E. Pecchioli, H.C. Hauffe, V. Tagliapietra, C. Bandi, C. Genchi, A. Rizzoli
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