Characterization of paucibacillary ileal lesions in sheep with subclinical active infection by Mycobacterium avium subsp. paratuberculosis
Articolo
Data di Pubblicazione:
2018
Citazione:
Characterization of paucibacillary ileal lesions in sheep with subclinical active infection by Mycobacterium avium subsp. paratuberculosis / S. Pisanu, T. Cubeddu, C. Cacciotto, Y. Pilicchi, D. Pagnozzi, S. Uzzau, S. Rocca, M.F. Addis. - In: VETERINARY RESEARCH. - ISSN 0928-4249. - 49:1(2018 Dec 04), pp. 117.1-117.15. [10.1186/s13567-018-0612-0]
Abstract:
Paratuberculosis (PTB) or Johne’s disease is a contagious enteritis of ruminants caused by Mycobacterium avium subsp.
paratuberculosis (MAP). Ovine PTB is less understood than bovine PTB, especially concerning paucibacillary infection
and its evolution into clinical disease. We combined shotgun proteomics, histopathology and immunohistochemistry
for the characterization of ileal tissues collected from seven asymptomatic sheep negative to serum ELISA, positive to
feces and tissue MAP IS900 and F57 PCR, histologically classified as paucibacillary, actively infected, together with 3
MAP-free controls (K). Following shotgun proteomics with label-free quantitation and differential analysis, 96 proteins
were significantly changed in PTB vs K, and were mostly involved in immune defense processes and in the macrophage-
MAP interaction. Principal component analysis (PCA) of protein abundances highlighted two PTB sample
clusters, PTB1 and PTB2, indicating a dichotomy in their proteomic profiles. This was in line with the PCA of histopathology
data and was related to features of type 2 (PTB1) and type 3a (PTB2) lesions, respectively. PTB2 proteomes differed
more than PTB1 proteomes from K: 43 proteins changed significantly only in PTB2 and 11 only in PTB1. The differential
proteins cathelicidin, haptoglobin, S100A8 and S100A9 were evaluated by immunohistochemistry. K tissues
were negative to cathelicidin and haptoglobin and sparsely positive to S100A8 and S100A9. PTB tissues were positive
to all four proteins, with significantly more cells in PTB2 than in PTB1. In conclusion, we described several pathways
altered in paucibacillary PTB, highlighted some proteomic differences among paucibacillary PTB cases, and identified
potential markers for disease understanding, staging, and detection.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
S. Pisanu, T. Cubeddu, C. Cacciotto, Y. Pilicchi, D. Pagnozzi, S. Uzzau, S. Rocca, M.F. Addis
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