Data di Pubblicazione:
2014
Citazione:
Persistent super-diffusive motion of Escherichia coli chromosomal loci / A. Javer, N.J. Kuwada, Z. Long, V.G. Benza, K.D. Dorfman, P.A. Wiggins, P. Cicuta, M. Cosentino Lagomarsino. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 5(2014 May 29). [10.1038/ncomms4854]
Abstract:
The physical nature of the bacterial chromosome has important implications for its function. Using high-resolution dynamic tracking, we observe the existence of rare but ubiquitous 'rapid movements' of chromosomal loci exhibiting near-ballistic dynamics. This suggests that these movements are either driven by an active machinery or part of stress-relaxation mechanisms. Comparison with a null physical model for subdiffusive chromosomal dynamics shows that rapid movements are excursions from a basal subdiffusive dynamics, likely due to driven and/or stress-relaxation motion. Additionally, rapid movements are in some cases coupled with known transitions of chromosomal segregation. They do not co-occur strictly with replication, their frequency varies with growth condition and chromosomal coordinate, and they show a preference for longitudinal motion. These findings support an emerging picture of the bacterial chromosome as off-equilibrium active matter and help developing a correct physical model of its in vivo dynamic structure.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Chromosome Segregation; Chromosomes, Bacterial; DNA Replication; Escherichia coli; Genetic Loci; Motion
Elenco autori:
A. Javer, N.J. Kuwada, Z. Long, V.G. Benza, K.D. Dorfman, P.A. Wiggins, P. Cicuta, M. Cosentino Lagomarsino
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