Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes
Articolo
Data di Pubblicazione:
2018
Citazione:
Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes / E. Onelli, M. Scali, M. Caccianiga, N. Stroppa, P. Morandini, G. Pavesi, A. Moscatelli. - In: OPEN BIOLOGY. - ISSN 2046-2441. - 8:10(2018 Oct). [10.1098/rsob.180078]
Abstract:
Fine regulation of exocytosis and endocytosis plays a basic role in pollen tube growth. Excess plasma membrane secreted during pollen tube elongation is known to be retrieved by endocytosis and partially reused in secretory pathways through the Golgi apparatus. Dissection of endocytosis has enabled distinct degradation pathways to be identified in tobacco pollen tubes and has shown that microtubules influence the transport of plasma membrane internalized in the tip region to vacuoles. Here, we used different drugs affecting the polymerization state of microtubules together with SYP21, a marker of prevacuolar compartments, to characterize trafficking of prevacuolar compartments in Nicotiana tabacum pollen tubes. Ultrastructural and biochemical analysis showed that microtubules bind SYP21-positive microsomes. Transient transformation of pollen tubes with LAT52-YFP-SYP21 revealed that microtubules play a key role in the delivery of prevacuolar compartments to tubular vacuoles.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
microtubules, prevacuolar compartment,
pollen tube, Nicotiana tabacum, vacuole
Elenco autori:
E. Onelli, M. Scali, M. Caccianiga, N. Stroppa, P. Morandini, G. Pavesi, A. Moscatelli
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