Expression analysis of anthocyanin regulatory genes in response to different light qualities in Arabidopsis thaliana
Articolo
Data di Pubblicazione:
2008
Citazione:
Expression analysis of anthocyanin regulatory genes in response to different light qualities in Arabidopsis thaliana / E. Cominelli, G. Gusmaroli, D. Allegra, M. Galbiati, H.K. Wade, G.I. Jenkins, C. Tonelli. - In: JOURNAL OF PLANT PHYSIOLOGY. - ISSN 0176-1617. - 165:8(2008), pp. 886-894.
Abstract:
In this work we analysed, at the transcript level, the response of Arabidopsis
anthocyanin regulatory genes of the MYB (PAP1 and PAP2), bHLH (TT8, EGL3 and GL3)
and WD40 (TTG1) families to white light in seedlings and to different light qualities in
rosette leaves. Our experiments showed strong light induction of the MYB genes PAP1
and PAP2. In particular, the kinetics of PAP1 expression preceded those of PAP2 and all
of the structural genes (CHS, DFR, F3H, LDOX), consistent with the hypothesis that it
has a key role in light induction of anthocyanin biosynthesis. All bHLH genes analysed
showed light induction, and in seedlings their expression preceded that of the late
structural genes, suggesting their possible role in light regulation of these structural
genes. TTG1 expression is essentially constitutive in both systems. Experiments with
transgenic lines over-expressing the MYB factors show that PAP1, but not PAP2, strongly
stimulates expression of the anthocyanin structural gene encoding dihydroflavonol
reductase, but neither factor affected expression of the early flavonoid biosynthesis
gene encoding chalcone synthase. Consistent with these findings, PAP1, but not PAP2,
stimulated light induction of anthocyanin biosynthesis in seedlings. We conclude that
specific members of the MYB and bHLH families play important roles in regulating
anthocyanin biosynthesis in response to different light qualities in Arabidopsis.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Anthocyanins ; Arabidopsis ; bHLH ; Light regulation ; MYB
Elenco autori:
E. Cominelli, G. Gusmaroli, D. Allegra, M. Galbiati, H.K. Wade, G.I. Jenkins, C. Tonelli
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