Effects of human recombinant type I IFNs (IFN-α2b and IFN-β1a) on growth and migration of primary endometrial stromal cells from women with deeply infiltrating endometriosis : a preliminary study
Articolo
Data di Pubblicazione:
2018
Citazione:
Effects of human recombinant type I IFNs (IFN-α2b and IFN-β1a) on growth and migration of primary endometrial stromal cells from women with deeply infiltrating endometriosis : a preliminary study / A. Dicitore, S. Castiglioni, D. Saronni, D. Gentilini, M.O. Borghi, S. Stabile, M. Vignali, A.M. Di Blasio, L. Persani, G. Vitale. - In: EUROPEAN JOURNAL OF OBSTETRICS, GYNECOLOGY, AND REPRODUCTIVE BIOLOGY. - ISSN 0301-2115. - 230(2018), pp. 192-198. [10.1016/j.ejogrb.2018.10.004]
Abstract:
Objective(s): Endometriosis is a major cause of infertility and disability for women, caused by the
presence of inflammatory endometrial implants in extrauterine locations. Among the constituents
involved in the immune response during the development of endometriosis, several chemokines,
including interferons (IFNs) may have a role in the pathogenesis of this disease. The aim of this
preliminary study was to investigate the anti-proliferative and anti-migratory activities of type I IFNs
(IFN-α2b and IFN-β1a) in primary endometrial stromal cells (ESCs) isolated from women with deeply
infiltrating endometriosis (DIE).
Study Design: The study subjects included 7 women ranged in the age from 27 to 37 years with diagnosis
of DIE (Stage III and IV). Collected primary ESC monolayers, isolated from endometriotic nodules, were
incubated with various concentrations (from 1 to 1000 IU/ml) of IFN-α2b or IFN-β1a.
Result(s): IFN-β1a had a significantly higher activity in hampering the proliferation of cells compared to
IFN-α2b. This effect could be related to the induction of apoptosis and cell cycle arrest in S phase,
observed in ESCs during incubation with IFN-β1a. Moreover, IFN-β1a was more potent than IFN-α2b in
inhibiting migration and EGF-induced ERK activity of primary ESCs.
Conclusion(s): The inhibitory in vitro effect on ESC proliferation and migration of IFN-β1a was much more
potent than IFN-α2b. These preliminary data offer the rationale for future preclinical and clinical trials
using IFN-β1a as a new tool for the therapy and tertiary prevention in patients with DIE.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Endometriosis; Migration; Proliferation; Type I interferons
Elenco autori:
A. Dicitore, S. Castiglioni, D. Saronni, D. Gentilini, M.O. Borghi, S. Stabile, M. Vignali, A.M. Di Blasio, L. Persani, G. Vitale
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