Evaluation of boldenone formation and related steroids transformations in veal faeces by liquid chromatography/tandem mass spectrometry
Articolo
Data di Pubblicazione:
2008
Citazione:
Evaluation of boldenone formation and related steroids transformations in veal faeces by liquid chromatography/tandem mass spectrometry / F. Arioli, M.P. Gavinelli, M.L. Fracchiolla, A. Casati, M. Fidani, E. Ferrer, G. Pompa. - In: RAPID COMMUNICATIONS IN MASS SPECTROMETRY. - ISSN 0951-4198. - 22:2(2008), pp. 217-223.
Abstract:
It is established that bovine urine can result positive for boldenone and androstadienedione in consequence of faecal contamination. The simple transfer of steroids to urine is one minor aspect of faecal contamination. A high de novo production of steroids in faeces after deposition and in faeces-contaminated urine is almost certainly due to microbial activity, although the precursor compounds and transformations leading to the presence of these illegal steroids are unclear. We developed a simple in vitro method - incubation of faecal matter suspended in 0.9% saline - to induce steroid transformations in faeces, and analyzed the products by liquid chromatography/tandem mass spectrometry, without the need for prior extraction. Norethandrolone was the internal standard. The linearity (R2: 0.987-0.999), sensitivity (LODs: 0.3 to 1.0 ng/mL; LOQs: 1.0 to 3.0 ng/mL), precision (intra-day CVs: 2.6-8.2; inter-day CVs: 4.5-11.5) and accuracy (percentage recovery: 89-120%) were calculated for the studied steroids. Androstenedione, androstadienedione, α- and β-boldenone, testosterone and epitestosterone transformations were investigated. Mutual interconversion of steroids was observed, although 17β-hydroxy steroids had low stability compared with 17α-hydroxy and 17-keto steroids. The results suggest that this simple in vitro system may be an effective way of studying hormone transformations in faeces and, after analogue studies, in faeces-contaminated urine. Copyright
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
F. Arioli, M.P. Gavinelli, M.L. Fracchiolla, A. Casati, M. Fidani, E. Ferrer, G. Pompa
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