Tdp-25 Routing to Autophagy and Proteasome Ameliorates its Aggregation in Amyotrophic Lateral Sclerosis Target Cells
Articolo
Data di Pubblicazione:
2018
Citazione:
Tdp-25 Routing to Autophagy and Proteasome Ameliorates its Aggregation in Amyotrophic Lateral Sclerosis Target Cells / M.E. Cicardi, R. Cristofani, P. Rusmini, M. Meroni, V. Ferrari, G. Vezzoli, B. Tedesco, M. Piccolella, E. Messi, M. Galbiati, A. Boncoraglio, S. Carra, V. Crippa, A. Poletti. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 8:1(2018).
Abstract:
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that primarily affects motoneurons, while non-neuronal cells may contribute to disease onset and progression. Most ALS cases are characterized by the mislocalization and aggregation of the TAR DNA binding protein 43 (TDP- 43) in affected cells. TDP-43 aggregates contain C-terminal TDP-43 fragments of 35 kDa (TDP-35) and 25 kDa (TDP-25) and have been mainly studied in motoneurons, while little is currently known about their rate of accumulation and clearance in myoblasts. Here, we performed a comparative study in immortalized motoneuronal like (NSC34; i-motoneurons) cells and stabilized myoblasts (C2C12; s-myoblasts) to evaluate if these two cell types differentially accumulate and clear TDP forms. The most aggregating specie in i-motoneurons is the TDP-25 fragment, mainly constituted by the “prion-like” domain of TDP-43. To a lower extent, TDP-25 also aggregates in s-myoblasts. In both cell types, all TDP species are cleared by proteasome, but TDP-25 impairs autophagy. Interestingly, the routing of TDP-25 fragment to proteasome, by overexpressing BAG1, or to autophagy, by overexpressing HSPB8 or BAG3 decreased its accumulation in both cell types. These results demonstrate that promoting the chaperone-assisted clearance of ALS-linked proteins is beneficial not only in motoneurons but also in myoblasts.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
HSPB8; autophagy; protein misfolding; aggregation; neurodegeneration; proteasome; ALS; TDP43; trehalose
Elenco autori:
M.E. Cicardi, R. Cristofani, P. Rusmini, M. Meroni, V. Ferrari, G. Vezzoli, B. Tedesco, M. Piccolella, E. Messi, M. Galbiati, A. Boncoraglio, S. Carra, V. Crippa, A. Poletti
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