Antisense reduction of thylakoidal ascorbate peroxidase in Arabidopsis enhances Paraquat-induced photooxidative stress and Nitric Oxide-induced cell death
Articolo
Data di Pubblicazione:
2005
Citazione:
Antisense reduction of thylakoidal ascorbate peroxidase in Arabidopsis enhances Paraquat-induced photooxidative stress and Nitric Oxide-induced cell death / D. Tarantino, C. Vannini, M. Bracale, M. Campa, C. Soave, I. Murgia. - In: PLANTA. - ISSN 0032-0935. - 221:6(2005), pp. 757-765.
Abstract:
The production and characterization of Arabidopsis plants containing a transgene in which the Arabidopsis tAPX is inserted in antisense orientation, is described. tAPX activity in these transgenic tAPX plants is around 50% of control level. The tAPX antisense plants are phenotypically indistinguishable from control plants under normal growth conditions; they show, however, enhanced sensitivity to the O < Stack >(2) < Superscript >-supercript stop -generating herbicide, Paraquat. Interestingly, the tAPX antisense plants show enhanced symptoms of damage when cell death is triggered through treatment with the nitric oxide-donor, SNP. These results are in accordance with the ones recently obtained with transgenic plants overexpressing tAPX; altogether, they suggest that tAPX, besides the known ROS scavenging role, is also involved in the fine changes of H2O2 concentration during signaling events.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
antisense; Arabidopsis thaliana; ascorbate peroxidase; nitric oxide; oxidative stress; chloroplasts
Elenco autori:
D. Tarantino, C. Vannini, M. Bracale, M. Campa, C. Soave, I. Murgia
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