Determination of Tebuconazole and Penconazole Fungicides in Rat and Human Hair by Liquid Chromatography/Tandem Mass Spectrometry
Articolo
Data di Pubblicazione:
2018
Citazione:
Determination of Tebuconazole and Penconazole Fungicides in Rat and Human Hair by Liquid Chromatography/Tandem Mass Spectrometry / E. Polledri, R. Mercadante, S. Fustinoni. - In: RAPID COMMUNICATIONS IN MASS SPECTROMETRY. - ISSN 0951-4198. - (2018 May 04). [Epub ahead of print] [10.1002/rcm.8153]
Abstract:
RATIONALE: Tebuconazole (TEB) and penconazole (PEN) are widely applied
fungicides and environmental contaminants; their toxicological properties include possible
effects to the unborn child, therefore the evaluation of human exposure is relevant to risk
assessment. Hair is a non-invasive specimen that incorporates pollutants allowing an extended
exposure window to be surveyed. Aim of this work was to develop and validate an assay for
the determination of TEB and PEN in human hair.
METHODS: Under optimised conditions, analytes were extracted soaking hair in
acetonitrile, in the presence of deuterated analogues, under heating and agitation. Chemical
separation was achieved using a C18 reversed-phase chromatographic column and detection
and quantification were performed, after a positive electrospray ionization, by triple
quadrupole mass spectrometry operating in selected reaction monitoring mode.
RESULTS: The assay validation showed a linear dynamic range up to 5 µg/L or 200
pg/mg hair, inter- and intra-run precisions <6%, and accuracies within 5% of spiked
concentrations. Limits of quantification were 0.001 µg/L or 1 pg/mg hair for both TEB and
PEN. Matrix effect experiments showed that the isotope dilution approach allowed for the
control of bias sources. TEB and PEN were determined in hair of rats exposed to a low dose
of TEB and in hair of agricultural workers exposed to TEB and/or PEN during the application
season, indicating that both chemicals are incorporated into the hair upon exposure.
CONCLUSIONS: The results of this study indicate that the developed assay is useful to
evaluate the exposure to TEB and PEN in humans.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
E. Polledri, R. Mercadante, S. Fustinoni
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