Molecular-Type Specific Multiplex PCR produces a distinct VNII PCR pattern among Cryptococcus neoformans species complex
Articolo
Data di Pubblicazione:
2018
Citazione:
Molecular-Type Specific Multiplex PCR produces a distinct VNII PCR pattern among Cryptococcus neoformans species complex / M. Cogliati, M.R. Andrianarivelo, M. Ellabib, E.N. Nnadi, M. Cornet. - In: MEDICAL MYCOLOGY. - ISSN 1369-3786. - (2018 May). [Epub ahead of print] [10.1093/mmy/myy022]
Abstract:
Prevalence of molecular type VNII among Cryptococcus neoformans species complex is probably underestimated since it can be distinguished from VNI only using molecular typing methods such as URA5-RFLP, AFLP, MLST, or whole genome sequencing. Previously, we described a multiplex polymerase chain reaction (PCR) method able to identify VNI, VNIV, and VNIII hybrids, but, at that time, VNII molecular type was not described yet. In this study, 16 VNII global isolates were analyzed by our multiplex PCR method, and results showed that it was able to produce a specific pattern for all the studied VNII isolates, which was different from those of VNI, VNIV, and VNIII.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cryptococcus neoformans species complex; molecular typing; molecular type VNII; MLST; multiplex PCR
Elenco autori:
M. Cogliati, M.R. Andrianarivelo, M. Ellabib, E.N. Nnadi, M. Cornet
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