Transcriptional role of androgen receptor in the expression of long non-coding RNA Sox2OT in neurogenesis
Articolo
Data di Pubblicazione:
2017
Citazione:
Transcriptional role of androgen receptor in the expression of long non-coding RNA Sox2OT in neurogenesis / V. Tosetti, J. Sassone, A.L.M. Ferri, M. Taiana, G. Bedini, S. Nava, G. Brenna, C. Di Resta, D. Pareyson, A.M. Di Giulio, S. Carelli, E.A. Parati, A. Gorio. - In: PLOS ONE. - ISSN 1932-6203. - 12:7(2017 Jul 12), pp. e0180579.1-e0180579.20. [10.1371/journal.pone.0180579]
Abstract:
The complex architecture of adult brain derives from tightly regulated migration
and differentiation of precursor cells generated during embryonic neurogenesis.
Changes at transcriptional level of genes that regulate migration and
differentiation may lead to neurodevelopmental disorders. Androgen receptor (AR)
is a transcription factor that is already expressed during early embryonic days.
However, AR role in the regulation of gene expression at early embryonic stage is
yet to be determinate. Long non-coding RNA (lncRNA) Sox2 overlapping transcript
(Sox2OT) plays a crucial role in gene expression control during development but
its transcriptional regulation is still to be clearly defined. Here, using
Bicalutamide in order to pharmacologically inactivated AR, we investigated
whether AR participates in the regulation of the transcription of the
lncRNASox2OTat early embryonic stage. We identified a new DNA binding region
upstream of Sox2 locus containing three androgen response elements (ARE), and
found that AR binds such a sequence in embryonic neural stem cells and in mouse
embryonic brain. Our data suggest that through this binding, AR can promote the
RNA polymerase II dependent transcription of Sox2OT. Our findings also suggest
that AR participates in embryonic neurogenesis through transcriptional control of
the long non-coding RNA Sox2OT.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
V. Tosetti, J. Sassone, A.L.M. Ferri, M. Taiana, G. Bedini, S. Nava, G. Brenna, C. Di Resta, D. Pareyson, A.M. Di Giulio, S. Carelli, E.A. Parati, A. Gorio
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