EPO-releasing neural precursor cells promote axonal regeneration and recovery of function in spinal cord traumatic injury
Articolo
Data di Pubblicazione:
2017
Citazione:
EPO-releasing neural precursor cells promote axonal regeneration and recovery of function in spinal cord traumatic injury / S. Carelli, T. Giallongo, Z. Gombalova, D. Merli, A.M. Di Giulio, A. Gorio. - In: RESTORATIVE NEUROLOGY AND NEUROSCIENCE. - ISSN 0922-6028. - 35:6(2017 Nov 21), pp. 583-599. [10.3233/RNN-170750]
Abstract:
BACKGROUND: Spinal cord injury (SCI) is a debilitating condition characterized by
a complex of neurological dysfunctions ranging from loss of sensation to partial
or complete limb paralysis. Recently, we reported that intravenous administration
of neural precursors physiologically releasing erythropoietin (namely Er-NPCs)
enhances functional recovery in animals following contusive spinal cord injury
through the counteraction of secondary degeneration. Er-NPCs reached and
accumulated at the lesion edges, where they survived throughout the prolonged
period of observation and differentiated mostly into cholinergic neuron-like
cells.
OBJECTIVE: The aim of this study was to investigate the potential reparative and
regenerative properties of Er-NPCs in a mouse experimental model of traumatic
spinal cord injury.
METHODS AND RESULTS: We report that Er-NPCs favoured the preservation of axonal
myelin and strongly promoted the regrowth across the lesion site of monoaminergic
and chatecolaminergic fibers that reached the distal portions of the injured
cord. The use of an anterograde tracer transported by the regenerating axons
allowed us to assess the extent of such a process. We show that axonal
fluoro-ruby labelling was practically absent in saline-treated mice, while it
resulted very significant in Er-NPCs transplanted animals.
CONCLUSION: Our study shows that Er-NPCs promoted recovery of function after
spinal cord injury, and that this is accompanied by preservation of myelination
and strong re-innervation of the distal cord. Thus, regenerated axons may have
contributed to the enhanced recovery of function after SCI.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
S. Carelli, T. Giallongo, Z. Gombalova, D. Merli, A.M. Di Giulio, A. Gorio
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