Corrigendum: Intronless WNT10B-short variant underlies new recurrent allele-specific rearrangement in acute myeloid leukaemia (Scientific Reports (2017) 6 (37201) DOI: 10.1038/srep37201)
Articolo
Data di Pubblicazione:
2017
Citazione:
Corrigendum: Intronless WNT10B-short variant underlies new recurrent allele-specific rearrangement in acute myeloid leukaemia (Scientific Reports (2017) 6 (37201) DOI: 10.1038/srep37201) / F. Lazzaroni, L. Del Giacco, D. Biasci, M. Turrini, L. Prosperi, R. Brusamolino, R. Cairoli, A. Beghini. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 7(2017 Apr 26). [10.1038/srep46788]
Abstract:
This Article contains typographical errors in the Methods section under subheading 'WNT10B/WNT10BIVS1 Gene expression analysis'. "The WNT10B (P4-P2 primers) amplification was performed with following thermal conditions: 94 °C for 1 min, 33 cycles at 94° for 30 s, 58 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min. The amplification of WNT10BIVS1 (P3-P2 primers) was performed as follows: 94 °C for 1 min, 33 cycles at 94° for 30 s, 61 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min". should read: "The WNT10B (P4-P1 primers) amplification was performed with following thermal conditions: 94 °C for 1 min, 33 cycles at 94° for 30 s, 58 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min. The amplification of WNT10BIVS1 (P3-P1 primers) was performed as follows: 94 °C for 1 min, 33 cycles at 94° for 30 s, 61 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min". In the same section, under subheading 'WNT10B/WNT10BIVS1 Absolute quantification', "We performed the experiment on Bio-Rad's QX100 ddPCR system and the reaction mixtures in a final 20 μ l volume consisted of 10 μ l of 2 × One-Step RT-ddPCR Supermix (Bio-Rad, CA, USA), 1 mM Manganese Acetate solution (Bio-Rad, CA, USA), 0.5 μ M of primers (WNT10B: P4-P2, WNT10BIVS1 P3-P2), 0.25 μ M WNT10B-dd1 and WNT10BIVS1-dd2 probes". should read: "We performed the experiment on Bio-Rad's QX100 ddPCR system and the reaction mixtures in a final 20 μ l volume consisted of 10 μ l of 2 × One-Step RT-ddPCR Supermix (Bio-Rad, CA, USA), 1 mM Manganese Acetate solution (Bio-Rad, CA, USA), 0.5 μ M of primers (WNT10B: P4-P1, WNT10BIVS1-P3-P1), 0.25 μ M WNT10B-dd1 and WNT10BIVS1-dd2 probes".
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
multidisciplinary
Elenco autori:
F. Lazzaroni, L. Del Giacco, D. Biasci, M. Turrini, L. Prosperi, R. Brusamolino, R. Cairoli, A. Beghini
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