Spermatozoa DNA and plasma membrane integrity after pellet optimized processing for cryopreservation in meat type chicken breeders
Articolo
Data di Pubblicazione:
2017
Citazione:
Spermatozoa DNA and plasma membrane integrity after pellet optimized processing for cryopreservation in meat type chicken breeders / T.M. Gliozzi, L. Zaniboni, N. Iaffaldano, S. Cerolini. - In: BRITISH POULTRY SCIENCE. - ISSN 0007-1668. - 58:5(2017), pp. 578-584.
Abstract:
1. Aim of this study was the development of an optimised cryopreservation pellet procedure for chicken semen and the assessment of DNA and membrane integrity in frozen/thawed spermatozoa in a Hubbard F15 meat type selected strain.2. The following semen processing conditions were studied: spermatozoa working concentration (SWC), 1.5 vs 2x10(9) cells/ml in pre-freezing extender; equilibration of diluted semen at 5 degrees C, 20 vs 40min; dimethylacetamide concentration, 6% vs 9%; dimethylacetamide equilibration time at 5 degrees C, 1 vs 30min; thawing at 60 degrees C for 10 vs 50 degrees C for 30sec. Spermatozoa viability (EtBr exclusion procedure - stress test), mobility (Accudenz (R) swim-down test) and subjective motility were assessed in fresh and frozen-thawed semen.3. The lower SWC (1.5 x 10(9) cells/ml) and the higher dimethylacetamide concentration (9%) had positive significant effects on the recovery rate of motile (22% vs 16%) and viable spermatozoa (39 vs 34%), respectively.4.Membrane (SYBR14-PI staining) and DNA integrity (comet assay) were assessed before and after freezing/thawing according to the optimised protocol.5. Recovery rates of spermatozoa with undamaged plasma membrane and DNA were 41% and 76%, respectively. The distribution of spermatozoa in classes of DNA damage was also analysed and discussed.6. It was concluded that pellet cryopreservation was a damaging process mainly for plasma membrane rather than nuclear DNA in chicken spermatozoa.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Chickens; DNA integrity; pellet cryopreservation; plasma membrane integrity; spermatozoa; Food Science; Animal Science and Zoology
Elenco autori:
T.M. Gliozzi, L. Zaniboni, N. Iaffaldano, S. Cerolini
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