Effectiveness of citrate buffer-fluoride mixture in terumo tubes as an inhibitor of in vitro glycolysis
Articolo
Data di Pubblicazione:
2016
Citazione:
Effectiveness of citrate buffer-fluoride mixture in terumo tubes as an inhibitor of in vitro glycolysis / G. Bonetti, M. Carta, M. Montagnana, C. Lo Cascio, A.R. Bonfigli, A. Mosca, R. Testa. - In: BIOCHEMIA MEDICA. - ISSN 1330-0962. - 26:1(2016 Feb), pp. 68-76. [10.11613/BM.2016.006]
Abstract:
Introduction: Glycolysis affects glucose determination in vitro. The placement of sample tubes in ice-water slurry with plasma separation within 30 minutes is recommended, or alternatively the use of a glycolysis inhibitor. The aim of our two-steps study was to evaluate which Terumo tube is best for glucose determination in routine clinical setting.Materials and methods: In the first study, blood from 100 volunteers was collected into lithium heparin (LH), NaF/Na heparin (FH) and NaF/citrate buffer/Na2EDTA (FC-Mixture) tubes. LH sample was treated as recommended and considered as reference, while FH and FC-Mixture samples were aliquoted, maintained at room temperature (RT) for 1, 2 and 4 hours; centrifuged and plasma analysed in triplicate. In the second study, samples from 375 volunteers were collected in LH, FH and FC-Mixture tubes and held at RT before centrifugation from 10 to 340 minutes, depending on each laboratory practice. Samples were analysed in one analytical run. Results: In the first study, FH glucose concentrations were 5.15 ± 0.66 mmol/L, 5.05 ± 0.65 mmol/L and 5.00 ± 0.65 mmol/L (P < 0.001) in tubes stored at RT for 1, 2 and 4 hours, respectively. Mean biases in all time points exceeded the analytical goal for desirable bias based on biological variation criteria. FC-Mixture glucose concentrations were 5.48 ± 0.65 mmol/L, 5.46 ± 0.6 mmol/L and 5.46 ± 0.64 mmol/L in tubes stored at RT for 1, 2 and 4 hours, respectively. Mean biases for FC-Mixture glucose in all time points reached optimal analytical goals. In the second study, the biases for LH and FH glucose compared to reference FC-Mixture glucose exceeded the preset analytical goals, regardless of the blood collection to centrifugation time interval. Conclusions: FC-mixture tubes glucose concentrations were preserved up to 4h storage at RT. We confirmed that NaF alone does not allow immediate glycolysis inhibition in real life pre-centrifugation storage conditions (up to 340 minutes). FC-Mixture should be used exclusively for glucose determination in laboratories unable to implement the recommended blood samples' treatment.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
citrate acidification; glucose; pre-analytical phase; sodium fluoride; stability; adolescent; adult; aged; anticoagulants; blood glucose; blood specimen collection; buffers; centrifugation; citrates; edetic acid; female; fluorides; glycolysis; heparin; humans; lithium; male; middle aged; reproducibility of results; temperature; time factors; young adult; clinical biochemistry; biochemistry (medical)
Elenco autori:
G. Bonetti, M. Carta, M. Montagnana, C. Lo Cascio, A.R. Bonfigli, A. Mosca, R. Testa
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