Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells
Articolo
Data di Pubblicazione:
2016
Citazione:
Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells / V. Bianchessi, M.C. Vinci, P. Nigro, V. Rizzi, F. Farina, M.C. Capogrossi, G. Pompilio, V. Gualdi, A. Lauri. - In: MITOCHONDRION. - ISSN 1567-7249. - 27(2016 Mar), pp. 40-47. [10.1016/j.mito.2016.02.004]
Abstract:
The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5mC clusters and a biased distribution of 5mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5mC in D-loop formation, rather than in mitochondrial gene expression.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bisulfite sequencing; D-loop; Endothelial Cell; Methylation; MtDNA; Senescence; Molecular Medicine; Molecular Biology; Cell Biology
Elenco autori:
V. Bianchessi, M.C. Vinci, P. Nigro, V. Rizzi, F.M. Farina, M.C. Capogrossi, G. Pompilio, V. Gualdi, A. Lauri
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