Derivation of the Duchennemuscular dystrophy patient-derived induced pluripotent stem cell line lacking DMD exons 49 and 50 (CCMi001DMD-A-3, Δ49, Δ50)
Articolo
Data di Pubblicazione:
2017
Citazione:
Derivation of the Duchennemuscular dystrophy patient-derived induced pluripotent stem cell line lacking DMD exons 49 and 50 (CCMi001DMD-A-3, Δ49, Δ50) / G. Spaltro, V. Vigorelli, F. Casalnuovo, P. Spinelli, E. Castiglioni, D. Rovina, S. Paganini, M. Di Segni, P. Nigro, C. Gervasini, G. Pompilio, A. Gowran. - In: STEM CELL RESEARCH. - ISSN 1873-5061. - 25(2017 Dec), pp. 128-131. [10.1016/j.scr.2017.10.018]
Abstract:
Duchenne muscular dystrophy (DMD) is caused by abnormalities in the dystrophin gene and is clinically characterised by childhood muscle degeneration and cardiomyopathy. We produced an induced pluripotent stem cell line from a DMD patient's dermal fibroblasts by electroporation with episomal vectors containing: hL-MYC, hLIN28, hSOX2, hKLF4, hOCT3/4. The resultant DMD iPSC line (CCMi001DMD-A-3) displayed iPSC morphology, expressed pluripotency markers, possessed trilineage differentiation potential and was karyotypically normal. MLPA analyses performed on DNA extracted from CCMi001DMD-A-3 showed a deletion of exons 49 and 50 (CCMi001DMD-A-3, ∆49, ∆50).
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Developmental Biology; Cell Biology
Elenco autori:
G. Spaltro, V. Vigorelli, F. Casalnuovo, P. Spinelli, E. Castiglioni, D. Rovina, S. Paganini, M. Di Segni, P. Nigro, C. Gervasini, G. Pompilio, A. Gowran
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