Data di Pubblicazione:
2017
Citazione:
LC-MS/MS Identification of Species-Specific Muscle Peptides in Processed Animal Proteins / D. Marchis, A. Altomare, M. Gili, F. Ostorero, A. Khadjavi, C. Corona, G. Ru, B. Cappelletti, S. Gianelli, F. Amadeo, C. Rumio, M. Carini, G. Aldini, C. Casalone. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - 65:48(2017), pp. 10638-10650. [10.1021/acs.jafc.7b04639]
Abstract:
An innovative analytical strategy has been applied to identify signature peptides able to distinguish among processed animal proteins (PAPs) derived from bovine, pig, fish, and milk products. Proteomics was first used to elucidate the proteome of each source. Starting from the identified proteins and using a funnel based approach, a set of abundant and well characterized peptides with suitable physical-chemical properties (signature peptides) and specific for each source was selected. An on-target LC-ESI-MS/MS method (MRM mode) was set up using standard peptides and was then applied to selectively identify the PAP source and also to distinguish proteins from bovine carcass and milk proteins. We believe that the method described meets the request of the European Commission which has developed a strategy for gradually lifting the "total ban" toward "species to species ban", therefore requiring official methods for species-specific discrimination in feed.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
bovine; bovine spongiform encephalopathy (BSE); fish; LC-QqLIT MS; pig; processed animal proteins (PAPs); signature peptides; Animals; Cattle; Chromatography, Liquid; Fishes; Meat; Milk; Muscle Proteins; Peptides; Proteomics; Species Specificity; Swine; Tandem Mass Spectrometry; Chemistry (all); Agricultural and Biological Sciences (all)
Elenco autori:
D. Marchis, A. Altomare, M. Gili, F. Ostorero, A. Khadjavi, C. Corona, G. Ru, B. Cappelletti, S. Gianelli, F. Amadeo, C. Rumio, M. Carini, G. Aldini, C. Casalone
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