Incorporation of Naked Peptide Nucleic Acids into Liposomes Leads to Fast and Efficient Delivery
Articolo
Data di Pubblicazione:
2015
Citazione:
Incorporation of Naked Peptide Nucleic Acids into Liposomes Leads to Fast and Efficient Delivery / C. Avitabile, A. Accardo, P. Ringhieri, G. Morelli, M. Saviano, G. Montagner, E. Fabbri, E. Gallerani, R. Gambari, A. Romanelli. - In: BIOCONJUGATE CHEMISTRY. - ISSN 1043-1802. - 26:8(2015), pp. 1533-1541. [10.1021/acs.bioconjchem.5b00156]
Abstract:
The delivery of peptide nucleic acids (PNAs) to cells is a very challenging task. We report here that a liposomal formulation composed of egg PC/cholesterol/DSPE-PEG2000 can be loaded, according to different encapsulation techniques, with PNA or fluorescent PNA oligomers. PNA loaded liposomes efficiently and quickly promote the uptake of a PNA targeting the microRNA miR-210 in human erythroleukemic K562 cells. By using this innovative delivery system for PNA, down-regulation of miR-210 is achieved at a low PNA concentration.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Down-Regulation; Gene Expression Regulation, Neoplastic; Humans; K562 Cells; MicroRNAs; Oligonucleotides, Antisense; Peptide Nucleic Acids; Phosphatidylethanolamines; Polyethylene Glycols; Drug Delivery Systems; Liposomes;
Elenco autori:
C. Avitabile, A. Accardo, P. Ringhieri, G. Morelli, M. Saviano, G. Montagner, E. Fabbri, E. Gallerani, R. Gambari, A. Romanelli
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