Data di Pubblicazione:
2004
Citazione:
Determination of Cryptococcus neoformans var. neoformans mating type by multiplex PCR / M.C. Esposto, M. Cogliati, A.M. Tortorano, M.A. Viviani. - In: CLINICAL MICROBIOLOGY AND INFECTION. - ISSN 1198-743X. - 10:12(2004), pp. 1092-1094.
Abstract:
Mating type plays an important role in the epidemiology and virulence of Cryptococcus neoformans. The present study designed a multiplex PCR method to distinguish the six mating type patterns (Aa, Da, Aalpha, Dalpha, Aa/Dalpha, and Aalpha/Da) of C. neoformans var. neoformans. PCR amplification identified one fragment for Aa (860 bp), Dalpha (413 bp) and Da (645 bp) strains, two fragments for Aalpha (320 and 400 bp) and Aa/Dalpha (860 and 413 bp) strains, and three fragments (645, 400, 320 bp) for an Aalpha/Da strain. The method appears to be a valid, simple and relatively inexpensive tool for epidemiological and virulence studies.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cryptococcus neoformans var. neoformans; Mating type patterns; PCR; Cryptococcus neoformans; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Virulence; Genes, Fungal; Genes, Mating Type, Fungal; Microbiology (medical); Infectious Diseases
Elenco autori:
M.C. Esposto, M. Cogliati, A.M. Tortorano, M.A. Viviani
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