Data di Pubblicazione:
2009
Citazione:
Neuropeptide Y overexpression using recombinant adenoassociated viral vectors / F. Noé, A. Frasca, C. Balducci, M. Carli, G. Sperk, F. Ferraguti, A. Pitkänen, R. Bland, H. Fitzsimons, M. During, A. Vezzani. - In: NEUROTHERAPEUTICS. - ISSN 1933-7213. - 6:2(2009 Apr 06), pp. 300-306. ((Intervento presentato al 4. convegno Workshop on new horizons in the development of antiepileptic drugs: nontraditional approaches to treat epilepsy tenutosi a Dublin (Ireland) nel 2008.
Abstract:
Gene therapy may represent a promising alternative treatment of epileptic patients who are resistant to conventional anti-epileptic drugs. Among the various approaches for the application of gene therapy in the treatment of CNS disorders, recombinant adeno-associated viral (AAV) vectors have been most widely used. Preclinical studies using a selection of "therapeutic" genes injected into the rodent brain to correct the compromised balance between inhibitory and excitatory transmission in epilepsy, showed significant reduction of seizures and inhibition of epileptogenesis. In particular, transduction of neuropeptide genes, such as galanin and neuropeptide Y (NPY) in specific brain areas in experimental models of seizures resulted in significant anticonvulsant effects. Recent findings showed a long-lasting NPY over-expression in the rat hippocampus by local application of recombinant AAV vectors associated with reduced generalization of seizures, delayed kindling epileptogenesis, and strong reduction of chronic spontaneous seizures. These results establish a proof-of-principle evidence of the efficacy of gene therapy as anticonvulsant treatment. Additional investigations are required to address safety concerns and possible side effects in more detail.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
adeno-associated viral vectors; anticonvulsant; gene therapy in epilepsy; neuropeptides; temporal lobe epilepsy; adenoviridae; animals; DNA, recombinant; epilepsy; genetic therapy; humans; neuropeptide Y; genetic vectors; pharmacology; neurology (clinical); pharmacology (medical)
Elenco autori:
F. Noé, A. Frasca, C. Balducci, M. Carli, G. Sperk, F. Ferraguti, A. Pitkänen, R. Bland, H. Fitzsimons, M. During, A. Vezzani
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