Cofilin is a cAMP effector in mediating actin cytoskeleton reorganization and steroidogenesis in mouse and human adrenocortical tumor cells
Articolo
Data di Pubblicazione:
2017
Citazione:
Cofilin is a cAMP effector in mediating actin cytoskeleton reorganization and steroidogenesis in mouse and human adrenocortical tumor cells / E. Peverelli, R. Catalano, E. Giardino, D. Treppiedi, V. Morelli, C.L. Ronchi, A. Vaczlavik, N. Fusco, S. Ferrero, J. Bertherat, F. Beuschlein, I. Chiodini, M. Arosio, A. Spada, G. Mantovani. - In: CANCER LETTERS. - ISSN 0304-3835. - 406:(2017 Oct), pp. 54-63. [10.1016/j.canlet.2017.07.025]
Abstract:
cAMP pathway plays a major role in the pathogenesis of cortisol-producing adrenocortical adenomas (CPA). cAMP-induced steroidogenesis is preceded by actin cytoskeleton reorganization, a process regulated by cofilin activity. In this study we investigated cofilin role in mediating cAMP effects on cell morphology and steroidogenesis in adrenocortical tumor cells. We demonstrated that forskolin induced cell rounding and strongly reduced phosphorylated (P)-cofilin/total cofilin ratio in Y1 (â52 ± 16%, p < 0.001) and human CPA cells (â53 ± 18%, p < 0.05). Cofilin silencing significantly reduced both forskolin-induced morphological changes and progesterone production (1.3-fold vs 1.8-fold in controls, p < 0.05), whereas transfection of wild-type or S3A (active), but not S3D (inactive) cofilin, potentiated forskolin effects on cell rounding and increased 3-fold progesterone synthesis with respect to control (p < 0.05). Furthermore, cofilin dephosphorylation by a ROCK inhibitor potentiated forskolin-induced cell rounding and steroidogenesis (2-fold increase vs forskolin alone). Finally, we found a reduced P-cofilin/total cofilin ratio and increased cofilin expression in CPA vs endocrine inactive adenomas by western blot and immunohistochemistry. Overall, these results identified cofilin as a mediator of cAMP effects on both morphological changes and steroidogenesis in mouse and human adrenocortical tumor cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Adrenocortical adenomas; cAMP; Cofilin; Cortisol; Cytoskeleton; Actin Cytoskeleton; Actin Depolymerizing Factors; Adrenal Cortex Neoplasms; Adrenocortical Adenoma; Animals; Colforsin; Cyclic AMP; Humans; Hydrocortisone; Mice; Phosphorylation; RNA, Small Interfering; Steroids; Tumor Cells, Cultured; Vasodilator Agents; Oncology; Cancer Research
Elenco autori:
E. Peverelli, R. Catalano, E. Giardino, D. Treppiedi, V. Morelli, C.L. Ronchi, A. Vaczlavik, N. Fusco, S. Ferrero, J. Bertherat, F. Beuschlein, I. Chiodini, M. Arosio, A. Spada, G. Mantovani
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