An early developmental vertebrate model for nanomaterial safety: bridging cell-based and mammalian toxicity assessment
Articolo
Data di Pubblicazione:
2016
Citazione:
An early developmental vertebrate model for nanomaterial safety: bridging cell-based and mammalian toxicity assessment / C. Webster, D. Di Silvio, A. Devarajan, P. Bigini, E. Micotti, C. Giudice, M. Salmona, G. Wheeler, V. Sherwood, F. Bombelli. - In: NANOMEDICINE. - ISSN 1743-5889. - 11:6(2016 Mar), pp. 643-656.
Abstract:
Aim: With the rise in production of nanoparticles (NPs) for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index. Results: We observed that, after exposure to a range of NPs, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored NPs, negative for toxicity in vitro and Xenopus, were further confirmed as nontoxic in mice. Conclusion: The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of NPs, which could be introduced for the routine testing of nanomaterials.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
cytotoxicity; in vitro-in vivo correlations; nanomaterials; nanomedicine; nanotoxicity; Xenopus laevis
Elenco autori:
C. Webster, D. Di Silvio, A. Devarajan, P. Bigini, E. Micotti, C. Giudice, M. Salmona, G. Wheeler, V. Sherwood, F. Bombelli
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