Matrix-assisted laser desorption mass spectrometry for the detection of recombinant bovine growth hormone in sustained-release form
Articolo
Data di Pubblicazione:
1995
Citazione:
Matrix-assisted laser desorption mass spectrometry for the detection of recombinant bovine growth hormone in sustained-release form / V. Borromeo, A. Berrini, C. Secchi, G.F. Brambilla, A. Cantafora. - In: JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS. - ISSN 0378-4347. - 669:2(1995 Jul 21), pp. 366-371.
Abstract:
We employed matrix-assisted laser desorption mass spectrometry (LD-MS) to detect recombinant bovine growth hormone (r-bGH) in sustained-release preparations. After preliminary extraction in phosphate buffer, LD-MS provided a precise determination of the molecular mass (Mr) of the r-bGH contained in 38 sustained-release preparations. The hormone was characterised using enzyme immunoassay, immunoblotting and amino acid sequencing. Rapid detection is essential for analysing large numbers of samples, and for monitoring the use of r-bGH in zootechnical productions and its administration as a "high-tech" drug for therapeutic purposes.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
chemistry (all)
Elenco autori:
V. Borromeo, A. Berrini, C. Secchi, G.F. Brambilla, A. Cantafora
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