Endopolygalacturonase Gene Polymorphisms : Asset of the Locus in Different Peach Accessions
Articolo
Data di Pubblicazione:
2017
Citazione:
Endopolygalacturonase Gene Polymorphisms : Asset of the Locus in Different Peach Accessions / S. Morgutti, N. Negrini, A. Ghiani, F. Baldin, D. Bassi, M. Cocucci. - In: AMERICAN JOURNAL OF PLANT SCIENCES. - ISSN 2158-2750. - 8:(2017 Mar 31), pp. 941-957. [10.4236/ajps.2017.84063]
Abstract:
Endopolygalacturonase (endoPG) plays a pivotal role in determining peach
[Prunus persica L. (Batsch)] fruit characteristics. Different Pp-endoPG genes or
allelic variants have been described, characterized by different polymorphisms:
insertions-deletions (InDels) and single nucleotide polymorphisms (SNPs).
Eighty-five peach accessions (comprising commercial cultivars, F1 progenies of
selected crosses, and three haploid seedlings) with different flesh softening patterns
(Non Melting: NM; Melting: M; Slow Softening: SS; Stony Hard: SH) were
screened by exploiting specific polymorphisms, with the aim to characterize
their asset at the endoPG locus and evaluate a potential relationship with fruit
flesh texture phenotype. The results of InDel analysis allowed to distinguish, by
a simple genotyping procedure, NM flesh phenotypes from the others. Further
information arose from this analysis, showing that two Pp-endoPG genes, i.e.,
Pp-endoPGm (Ppa006839m), involved in the determination of the Melting/Non
Melting trait, and Pp-endoPG_M (Ppa006857m), involved in the determination
of the Clingstone/Freestone trait, always co-segregate, and that SS Big Top possesses
a “null” Pp-endoPG allele. Cleaved Amplified Polymorphic Sequence
(CAPS) analysis allowed to preliminarily discriminate the Pp-endoPG variants
of the SS and SH accessions considered. The integrated use of the considered
polymorphisms in a high number of peach accessions proved useful,
by individuating the different gene variants and their combinations, to describe
the structure of the endoPG locus in different genotypes.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
CAPS Analysis, InDel/SNP Polymorphisms, Pp-endoPG, Prunus persica
Elenco autori:
S. Morgutti, N. Negrini, A. Ghiani, F. Baldin, D. Bassi, M. Cocucci
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