Semi-preparative HPLC purification of δ-tocotrienol (δ-T3) from Elaeis guineensis Jacq. and Bixa orellana L. and evaluation of its in vitro anticancer activity in human A375 melanoma cells
Articolo
Data di Pubblicazione:
2017
Citazione:
Semi-preparative HPLC purification of δ-tocotrienol (δ-T3) from Elaeis guineensis Jacq. and Bixa orellana L. and evaluation of its in vitro anticancer activity in human A375 melanoma cells / G. Beretta, F. Gelmini, F. Fontana, R.M. Moretti, M. Montagnani Marelli, P. Limonta. - In: NATURAL PRODUCT RESEARCH. - ISSN 1478-6419. - (2017 Apr 24), pp. 1-6. [Epub ahead of print] [10.1080/14786419.2017.1320793]
Abstract:
In this work, we report a rapid and convenient HPLC-UV-DAD method for the isolation of δ-T3 on semi-preparative scale from two different vitamin E rich processed, commercially available products obtained from the fruits of Elaeis guineensis Jacq. (oil palm) and from the seeds of Bixa orellana L. (achiote tree). Chromatography was run using reverse phase (RP) C-18 columns and HPLC-grade acetonitrile as mobile phase. The purity of the isolated δ-T3, assessed by GC-MS and (1)H NMR was above 98%. The δ-T3 cytotoxic activity found in vitro against the proliferation of human A375 melanoma cells compared to that of the other δ-T3 free tocols mixture suggest its primary role in the experimental anticancer activity observed for palm oil derived products. Taken altogether, the results of this study highlight the importance of the application of suitable purification systems for the preparations of tocotrienols prior to their experimental or clinical testing.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bixa orellana; Elaeis guineensis; semi-preparative HPLC; vitamin E; δ-tocotrienol
Elenco autori:
G. Beretta, F. Gelmini, F. Fontana, R.M. Moretti, M. Montagnani Marelli, P. Limonta
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