Application of 2D-DIGE to formalin-fixed diseased tissue samples from hospital repositories : Results from four case studies
Articolo
Data di Pubblicazione:
2013
Citazione:
Application of 2D-DIGE to formalin-fixed diseased tissue samples from hospital repositories : Results from four case studies / A. Tanca, S. Pisanu, G. Biosa, D. Pagnozzi, E. Antuofermo, G.P. Burrai, V. Canzonieri, P. Cossu-Rocca, V.D. Re, A. Eccher, G. Fanciulli, S. Rocca, S. Uzzau, M.F. Addis. - In: PROTEOMICS. CLINICAL APPLICATIONS. - ISSN 1862-8346. - 7:3-4(2013 Apr), pp. 252-263.
Abstract:
Purpose: In the recent past, the potential suitability of fixed samples to 2D-DIGE studies has been demonstrated on model tissues, but not on "real-world" archival tissues. Therefore, this study was aimed to assess the quality of the results delivered by 2D-DIGE on samples retrieved from hospital tissue repositories. Experimental design: Diseased and normal tissue samples (namely, human gastric adenocarcinoma and normal gastric tissue, human lung neuroendocrine tumors, canine mammary tubulo-papillary carcinoma and normal mammary tissue, sheep liver with cloudy swelling degeneration and normal liver tissue) were retrieved from human and veterinary biorepositories and subjected to full-length protein extraction, cyanine labeling, 2D-DIGE separation, image analysis, MS analysis, and protein identification. Results: Archival samples could be successfully subjected to 2D-DIGE, providing maps of satisfactory resolution, although with varying pattern complexity (possibly influenced by preanalytical variables). Moreover, differentially expressed protein identities were consistent with the disease biology. Conclusions and clinical relevance: 2D-DIGE can support biomarker discovery and validation studies on large sample cohorts. In fact, although some information complexity is lost when compared to fresh-frozen tissues, their vast availability and the associated patient information can considerably boost studies suffering limited sample availability or involving long-distance exchange of samples.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
canine mammary tumor; ffpe; gastric adenocarcinoma; gel-based proteomics; lung neuroendocrine tumor; animals; biomarkers; dogs; formaldehyde; humans; lung neoplasms; paraffin embedding; proteins; proteomics; sheep; small cell lung carcinoma; tissue banks; tissue fixation; two-dimensional difference gel electrophoresis; clinical biochemistry
Elenco autori:
A. Tanca, S. Pisanu, G. Biosa, D. Pagnozzi, E. Antuofermo, G.P. Burrai, V. Canzonieri, P. Cossu-Rocca, V.D. Re, A. Eccher, G. Fanciulli, S. Rocca, S. Uzzau, M.F. Addis
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