Data di Pubblicazione:
2014
Citazione:
Immunohistochemical tools and techniques to visualize notch in Drosophila melanogaster / E. Tognon, T. Vaccari (METHODS IN MOLECULAR BIOLOGY). - In: Notch Signaling : Methods and Protocols / [a cura di] H.J. Bellen, S. Yamamoto. - [s.l] : Humana Press Inc., 2014. - ISBN 9781493911387. - pp. 63-78 [10.1007/978-1-4939-1139-4_5]
Abstract:
The ability to accurately visualize proteins in Drosophila tissues is critical for studying their abundance and localization relative to the morphology of cells during tissue development and homeostasis. Here we describe the procedure to visualize Notch localization in whole-mount preparations of several Drosophila organs using confocal microscopy. The use of monoclonal antibodies directed to distinct portions of Notch allows one to follow the fate of the receptor during constitutive and inductive processes. The protocol described here can be used to co-label with antibodies recognizing markers of subcellular compartments in wild-type as well as mutant tissues.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
Notch localization; Drosophila melanogaster; Imaginal discs; Whole-mount immunohistochemistry; Confocal microscopy
Elenco autori:
E. Tognon, T. Vaccari
Link alla scheda completa:
Titolo del libro:
Notch Signaling : Methods and Protocols