The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in arabidopsis
Articolo
Data di Pubblicazione:
2010
Citazione:
The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in arabidopsis / M. Sugliani, V. Brambilla, E.J.M. Clerkx, M. Koornneef, W.J.J. Soppe. - In: PLANT CELL. - ISSN 1040-4651. - 22:6(2010), pp. 1936-1946. [10.1105/tpc.110.074674]
Abstract:
ABSCISIC ACID INSENSITIVE3 (ABI3) is a major regulator of seed maturation in Arabidopsis thaliana. We detected two ABI3 transcripts, ABI3-α and ABI3-β, which encode full-length and truncated proteins, respectively. Alternative splicing of ABI3 is developmentally regulated, and the ABI3-β transcript accumulates at the end of seed maturation. The two ABI3 transcripts differ by the presence of a cryptic intron in ABI3-α, which is spliced out in ABI3-β. The suppressor of abi3-5 (sua) mutant consistently restores wild-type seed features in the frameshift mutant abi3-5 but does not suppress other abi3 mutant alleles. SUA is a conserved splicing factor, homologous to the human protein RBM5, and reduces splicing of the cryptic ABI3 intron, leading to a decrease in ABI3-β transcript. In the abi3-5 mutant, ABI3-β codes for a functional ABI3 protein due to frameshift restoration. © 2010 American Society of Plant Biologists.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Arabidopsis; Arabidopsis Proteins; Base Sequence; Chromosome Mapping; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Germination; Introns; Molecular Sequence Data; Mutation; Phylogeny; RNA, Plant; RNA-Binding Proteins; Transcription Factors; Alternative Splicing; Plant Science; Cell Biology; Medicine (all)
Elenco autori:
M. Sugliani, V. Brambilla, E.J.M. Clerkx, M. Koornneef, W.J.J. Soppe
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