Microvesicles secreted from equine amniotic cells and their potential role in in vitro cell tendon repair
Abstract
Data di Pubblicazione:
2015
Citazione:
Microvesicles secreted from equine amniotic cells and their potential role in in vitro cell tendon repair / C. Perrini, A. Lange-Consiglio, R. Tasquier, M. Deregibus, G. Camussi, L. Pascucci, M.G. Marini, B. Corradetti, D. Bizzaro, F. Cremonesi. - In: INTERNATIONAL JOURNAL OF HEALTH, ANIMAL SCIENCE & FOOD SAFETY. - ISSN 2283-3927. - 2:suppl. 1(2015 Jul 17), pp. 1-1. ((Intervento presentato al convegno Veterinary and Animal Science Days tenutosi a Milano nel 2015 [10.13130/2283-3927/5104].
Abstract:
The regenerative mechanisms ascribed to mesenchymal stem cells (MSCs) are classified into 3 categories:
differentiating into damaged cell types, supplying nutrients, and improving survival/functions of the endogenous
cells via paracrine actions. However, because of the inhospitable microenvironment of the injured tissues, a
proportion of the implanted MSCs may quickly die, suggesting that other mechanisms might be present. This
notion is supported by the overlapping beneficial effect (in terms of time of healing) resulted after the injection
of AMCs or of amniotic mesenchymal cells - conditioned medium (AMC-CM) in equine spontaneous injured
tendons and ligaments. Microvesicles (MVs) released by cells are an integral component of the cell-to-cell
communication network involved in tissue regeneration.In the present study, MVs secreted by AMCs were
investigated with Nanosigth instrument and TEM. Then, the in vitro incorporation of MVs into equine tendon cells
was studied by a dose-response curve. Lastly, the ability of MVs to counteract an in vitro inflammatory process
induced by lipolysaccaride on tendon cells was studied evaluating the expression of pro-inflammatory genes like
metallopeptidase (MPP) 1 and 13, and prostaglandin-endoperoxide synthase 2 (COX2). Results demonstrated that
AMCs secreted MVs ranging in size from 100 to 1000 nm with a prevalence of 100-200 nm large MVs. Tendon cells
were able to uptake them with an inverse relationship between concentration and time. The greatest
incorporation was detectable at 40x106 MVs/ml after 72h. MVs induced down-regulation of MMP1 and MMP13,
suggesting that they may have contributed, along with soluble factors, to in vivo tendon regeneration.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
regenerative medicine; AMCs; MVs; tendon cells
Elenco autori:
C. Perrini, A. Lange-Consiglio, R. Tasquier, M. Deregibus, G. Camussi, L. Pascucci, M.G. Marini, B. Corradetti, D. Bizzaro, F. Cremonesi
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