Data di Pubblicazione:
2016
Citazione:
Direct non transcriptional role of NF-Y in DNA replication / P. Benatti, S. Belluti, B. Miotto, J. Neusiedler, D. Dolfini, M. Drac, V. Basile, E. Schwob, R. Mantovani, J.J. Blow, C. Imbriano. - In: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. - ISSN 0167-4889. - 1863:4(2016 Apr), pp. 673-685. [10.1016/j.bbamcr.2015.12.019]
Abstract:
NF-Y is a heterotrimeric transcription factor, which plays a pioneer role in the transcriptional control of promoters containing the CCAAT-box, among which genes involved in cell cycle regulation, apoptosis and DNA damage response. The knock-down of the sequence-specific subunit NF-YA triggers defects in S-phase progression, which lead to apoptotic cell death. Here, we report that NF-Y has a critical function in DNA replication progression, independent from its transcriptional activity. NF-YA colocalizes with early DNA replication factories, its depletion affects the loading of replisome proteins to DNA, among which Cdc45, and delays the passage from early to middle-late S phase. Molecular combing experiments are consistent with a role for NF-Y in the control of fork progression. Finally, we unambiguously demonstrate a direct non-transcriptional role of NF-Y in the overall efficiency of DNA replication, specifically in the DNA elongation process, using a Xenopus cell-free system. Our findings broaden the activity of NF-Y on a DNA metabolism other than transcription, supporting the existence of specific TFs required for proper and efficient DNA replication.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
CCAAT-binding factor; DNA replication; NF-Y; Transcription factors; Xenopus cell-free system; Animals; CCAAT-binding factor; cell cycle proteins; cells, cultured; DNA; DNA replication; HCT116 cells; humans; promoter regions, genetic; s phase; transcription elongation, genetic; transcription, genetic; xenopus laevis; cell biology; molecular biology; medicine (all)
Elenco autori:
P. Benatti, S. Belluti, B. Miotto, J. Neusiedler, D. Dolfini, M. Drac, V. Basile, E. Schwob, R. Mantovani, J.J. Blow, C. Imbriano
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