Preparation and immunogenicity of gold glyco-nanoparticles as antipneumococcal vaccine model
Articolo
Data di Pubblicazione:
2017
Citazione:
Preparation and immunogenicity of gold glyco-nanoparticles as antipneumococcal vaccine model / M. Vetro, D. Safari, S. Fallarini, K. Salsabila, M. Lahmann, S. Penadés, L. Lay, M. Marradi, F. Compostella. - In: NANOMEDICINE. - ISSN 1748-6963. - 12:1(2017 Jan), pp. 13-23.
Abstract:
Nanotechnology-based fully synthetic carbohydrate vaccines are promising alternatives to classic polysaccharide/protein conjugate vaccines. We have prepared gold glyco-nanoparticles (GNP) bearing two synthetic carbohydrate antigens related to serotypes 19F and 14 of Streptococcus pneumoniae and evaluated their immunogenicity in vivo. Results: A tetrasaccharide fragment of serotype 14 (Tetra-14), a trisaccharide fragment of serotype 19F (Tri-19F), a T-helper peptide and d -glucose were loaded onto GNP in different ratios. Mice immunization showed that the concomitant presence of Tri-19F and Tetra-14 on the same nanoparticle critically enhanced the titers of specific IgG antibodies toward type 14 polysaccharide compared with GNP exclusively displaying Tetra-14, while no IgG antibodies against type 19F polysaccharide were elicited. Conclusion: This work is a step forward toward synthetic nanosystems combining carbohydrate antigens and immunogenic peptides as potential carbohydrate-based vaccines.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
capsular polysaccharide fragments; carbohydrate-based vaccines; gold glyco-nanoparticles; immunogenicity; Streptococcus pneumoniae
Elenco autori:
M. Vetro, D. Safari, S. Fallarini, K. Salsabila, M. Lahmann, S. Penadés, L. Lay, M. Marradi, F. Compostella
Link alla scheda completa: