Validation of a quick PCR method suitable for direct sequencing : identification of Fusarium fungal species and chemotypes for preventive approaches in food safety
Articolo
Data di Pubblicazione:
2014
Citazione:
Validation of a quick PCR method suitable for direct sequencing : identification of Fusarium fungal species and chemotypes for preventive approaches in food safety / M. Pallez, M. Pasquali, T. Bohn, L. Hoffmann, M. Beyer. - In: FOOD TECHNOLOGY AND BIOTECHNOLOGY. - ISSN 1330-9862. - 52:3(2014), pp. 351-358.
Abstract:
Species determination by sequencing and PCR genetic chemotyping, used to determine the toxigenic potential of Fusarium strains, is fundamental for developing preventive strategies in food safety. Here we propose and statistically validate a quick protocol for standardizing the procedure of species determination by sequencing of the elongation factor 1-α and multiplex genetic chemotyping using the Tri12 gene, based on fungal growth on Miracloth tissue coupled with microwave extraction. The test was validated on 75 Fusarium culmorum and Fusarium graminearum strains.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
EF1-α; Fusarium culmorum; Fusarium graminearum; Microwave DNA extraction; Food Science; Industrial and Manufacturing Engineering; Chemical Engineering (all); Biotechnology
Elenco autori:
M. Pallez, M. Pasquali, T. Bohn, L. Hoffmann, M. Beyer
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