2D difference gel electrophoresis reference map of a Fusarium graminearum nivalenol producing strain
Articolo
Data di Pubblicazione:
2013
Citazione:
2D difference gel electrophoresis reference map of a Fusarium graminearum nivalenol producing strain / M. Pasquali, T. Serchi, J. Renaut, L. Hoffmann, T. Bohn. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 34:4(2013), pp. 505-509. [10.1002/elps.201200256]
Abstract:
Fusarium graminearum is widely studied as a model for toxin production among plant pathogenic fungi. A 2D DIGE reference map for the nivalenol-producing strain 453 was established. Based on a whole protein extract, all reproducible spots were systematically picked and analyzed by MALDI-TOF/TOF, leading to the identification of 1102 protein species. The obtained map contributes to the annotation of the genome by identifying previously nondescribed hypothetical proteins and will serve as a reference for future studies aiming at deciphering F. graminearum biology and chemotype diversity.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Fungal proteomics; Genome annotation; Proteome reference map; Trichothecene; Databases, Protein; Electrophoresis, Gel, Two-Dimensional; Fungal Proteins; Fusarium; Proteome; Reproducibility of Results; Trichothecenes; Biochemistry; Clinical Biochemistry
Elenco autori:
M. Pasquali, T. Serchi, J. Renaut, L. Hoffmann, T. Bohn
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