Transposition of the miniature inverted-repeat transposable element mimp1 in the wheat pathogen Fusarium culmorum
Articolo
Data di Pubblicazione:
2012
Citazione:
Transposition of the miniature inverted-repeat transposable element mimp1 in the wheat pathogen Fusarium culmorum / F. Spanu, M. Pasquali, B. Scherm, V. Balmas, A. Marcello, G. Ortu, M. Dufresne, L. Hoffmann, M. Daboussi, Q. Migheli. - In: MOLECULAR PLANT PATHOLOGY. - ISSN 1464-6722. - 13:9(2012), pp. 1149-1155. [10.1111/j.1364-3703.2012.00823.x]
Abstract:
High-throughput methods are needed for functional genomics analysis in Fusarium culmorum, the cause of crown and foot rot on wheat and a type B trichothecene producer. Our aim was to develop and test the efficacy of a double-component system based on the ability of the impala transposase to transactivate the miniature inverted-repeat transposable element mimp1 of Fusarium oxysporum. We report, for the first time, the application of a tagging system based on a heterologous transposon and of splinkerette-polymerase chain reaction to identify mimp1 flanking regions in the filamentous fungus F.culmorum. Similar to previous observations in Fusarium graminearum, mimp1 transposes in F.culmorum by a cut-and-paste mechanism into TA dinucleotides, which are duplicated on insertion. mimp1 was reinserted in open reading frames in 16.4% (i.e. 10 of 61) of the strains analysed, probably spanning throughout the entire genome of F.culmorum. The effectiveness of the mimp1/impala double-component system for gene tagging in F.culmorum was confirmed phenotypically for a putative aurofusarin gene. This system also allowed the identification of two genes putatively involved in oxidative stress-coping capabilities in F.culmorum, as well as a sequence specific to this fungus, thus suggesting the valuable exploratory role of this tool.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
blotting, southern; DNA transposable elements; fusarium; genes, fungal; inverted repeat sequences; mutagenesis, insertional; triticum; plant science; agronomy and crop science; soil science; molecular biology
Elenco autori:
F. Spanu, M. Pasquali, B. Scherm, V. Balmas, A. Marcello, G. Ortu, M. Dufresne, L. Hoffmann, M. Daboussi, Q. Migheli
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