Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex.
Articolo
Data di Pubblicazione:
2016
Citazione:
Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex / F. Bifari, I. Decimo, A. Pino, E. Llorens Bobadilla, S. Zhao, C. Lange, G. Panuccio, B. Boeckx, B. Thienpont, S. Vinckier, S. Wyns, A. Bouché, D. Lambrechts, M. Giugliano, M. Dewerchin, A. Martin Villalba, P. Carmeliet. - In: CELL STEM CELL. - ISSN 1934-5909. - (2016). [Epub ahead of print] [10.1016/j.stem.2016.10.020]
Abstract:
Whether new neurons are added in the postnatal cerebral cortex is still debated. Here, we report that the meninges of perinatal mice contain a population of neurogenic progenitors formed during embryonic development that migrate to the caudal cortex and differentiate into Satb2+ neurons in cortical layers II-IV. The resulting neurons are electrically functional and integrated into local microcircuits. Single-cell RNA sequencing identified meningeal cells with distinct transcriptome signatures characteristic of (1) neurogenic radial glia-like cells (resembling neural stem cells in the SVZ), (2) neuronal cells, and (3) a cell type with an intermediate phenotype, possibly representing radial glia-like meningeal cells differentiating to neuronal cells. Thus, we have identified a pool of embryonically derived radial glia-like cells present in the meninges that migrate and differentiate into functional neurons in the neonatal cerebral cortex.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
PDGFRβ; electrophysiology; lineage tracing; meninges; neonatal cerebral cortex; neural progenitors; neurogenesis; radial glia cells; single-cell RNA sequencing
Elenco autori:
F. Bifari, I. Decimo, A. Pino, E. Llorens Bobadilla, S. Zhao, C. Lange, G. Panuccio, B. Boeckx, B. Thienpont, S. Vinckier, S. Wyns, A. Bouché, D. Lambrechts, M. Giugliano, M. Dewerchin, A. Martin Villalba, P. Carmeliet
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