Data di Pubblicazione:
2017
Citazione:
Identification of novel loci for the generation of reporter mice / N. Rizzi, M. Rebecchi, G. Levandis, P. Ciana, A. Maggi. - In: NUCLEIC ACIDS RESEARCH. - ISSN 1362-4962. - 45:6(2017 Apr 07), pp. e37.1-e37.14. [10.1093/nar/gkw1142]
Abstract:
Deciphering the etiology of complex pathologies at molecular level requires longitudinal studies encompassing
multiple biochemical pathways (apoptosis, proliferation, inflammation, oxidative stress).
In vivo imaging of current reporter animals enabled the spatio-temporal analysis of specific molecular
events, however, the lack of a multiplicity of loci for the generalized and regulated expression of the integrated
transgenes hampers the creation of systems for the simultaneous analysis of more than a biochemical
pathways at the time. We here developed and tested an in vivo-based methodology for the
identification of multiple insertional loci suitable for the generation of reliable reporter mice. The validity
of the methodology was tested with the generation of novel mice useful to report on inflammation and
oxidative stress.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
molecular imaging; reporter mouse transgenesis; multiplex reporting in vivo; reporter systems; NFkB
Elenco autori:
N. Rizzi, M. Rebecchi, G. Levandis, P. Ciana, A. Maggi
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