Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois
Articolo
Data di Pubblicazione:
2015
Citazione:
Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois / C. De Liberato, F. Berrilli, M. Marangi, M. Santoro, T. Trogu, L. Putignani, P. Lanfranchi, F. Ferretti, S. D’Amelio, A. Giangaspero. - In: PARASITES & VECTORS. - ISSN 1756-3305. - 8(2015), pp. 650.1-650.7. [10.1186/s13071-015-1243-1]
Abstract:
Background: Although chamois Rupicapra spp. are the most abundant mountain ungulates in Europe, no data are
available on the presence of Giardia duodenalis infecting these species.
Methods: A total of 157 fecal samples from Alpine Rupicapra rupicapra rupicapra and Apennine Rupicapra pyrenaica
ornata chamois were tested for the presence of G. duodenalis by immunofluorescence test, quantitative Real Time
PCR and end-point PCR for genotype characterization.
Results: G. duodenalis was detected in R. r. rupicapra and R. p. ornata, with a percentage value of 4.45 (5.82 and
1.85 %, respectively), and a cyst burden of up to 31,800 cysts/g of feces. Assemblages A/AI and E were identified in
R. r. rupicapra and assemblage A/AIII in R. p. ornata.
Conclusions: The present study represents the first record of Giardia duodenalis in Rupicapra spp., suggesting that
these wild bovids can play an epidemiological role in environmental contamination and transmission of both
zoonotic and non-zoonotic genotypes.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Giardia duodenalis; Rupicapra rupicapra rupicapra; Rupicapra pyrenaica ornata; IF; qPCR; end-point-PCR
Elenco autori:
C. De Liberato, F. Berrilli, M. Marangi, M. Santoro, T. Trogu, L. Putignani, P. Lanfranchi, F. Ferretti, S. D’Amelio, A. Giangaspero
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