Prostaglandin E2 possesses different potencies in inducing Vascular Endothelial Growth Factor and Interleukin-8 production in COPD human lung fibroblasts
Articolo
Data di Pubblicazione:
2016
Citazione:
Prostaglandin E2 possesses different potencies in inducing Vascular Endothelial Growth Factor and Interleukin-8 production in COPD human lung fibroblasts / A. Bonanno, G.D. Albano, L. Siena, A.M. Montalbano, L. Riccobono, G. Anzalone, G. Chiappara, R. Gagliardo, M. Profita, A. Sala. - In: PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS. - ISSN 0952-3278. - 106:(2016 Mar), pp. 11-18. [10.1016/j.plefa.2016.01.005]
Abstract:
We studied the role of PGE2, its biosynthetic enzymes and its receptors, in regulating the functions of lung fibroblasts through the production of Vascular Endothelial Growth Factor (VEGF) and Interleukin-8 (IL-8) in COPD subjects. Lung fibroblasts from Control (C) (n=6), Smoker (HS) (n=6) and COPD patients (n=8) were cultured, and basal PGE2, VEGF, and IL-8 measured in supernatants by ELISA. COX-1/COX-2 and EP receptors expression were assessed by western blot and by RT-PCR. Release of VEGF and IL-8 by human fetal lung fibroblasts (HFL-1; lung, diploid, human) was evaluated under different conditions. PGE2, VEGF, and IL-8 levels, COX-2, EP2, and EP4 protein expression and mRNA were increased in COPD when compared to Controls. Low concentrations of synthetic PGE2 increased the release of VEGF in HFL-1, but higher concentrations were needed to induce the release of IL-8. This effect was mimicked by an EP2 agonist and modulated by an EP4 antagonist. In the airways of COPD subjects, fibroblast-derived PGE2 may regulate angiogenesis and inflammation through the production of VEGF and IL-8 respectively, suggesting that the increase in expression of COX-2, EP2 and EP4 observed in COPD fibroblasts may contribute to steering the role of PGE2 from homeostatic to pro-inflammatory.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
angiogenesis; COPD; cyclooxygenase-2; EP receptors; lung inflammation; prostaglandin E2; cell biology; clinical biochemistry
Elenco autori:
A. Bonanno, G.D. Albano, L. Siena, A.M. Montalbano, L. Riccobono, G. Anzalone, G. Chiappara, R. Gagliardo, M. Profita, A. Sala
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